20 August, 2020
As co-author, Zhadyra Yerkesh tells us about the fruitful collaboration with Prof. Dirk Schwarzer’s lab, and how they recently developed a novel experimental approach for establishing a ‘designer’ nucleosome library in a high-throughput fashion. This uncovered a previously unknown role for H3S28ph in enhancing the recruitment of HP1 protein to the H3K9me2 only in nucleosomal context. "My contribution to this work was to optimize the procedures for reconstitution of the ‘ligation-ready’ nucleosomes in a large scale. To independently verify the putative role of H3S28ph in HP1 recruitment, I carried out quantitative binding assays (MST, ITC) and prepared the nucleosome samples for NMR studies. It was a challenging yet exciting journey that resulted in my first co-authorship article. I hope that this novel method will serve well to the chromatin community and open doors to many discoveries yet to come!"
Congratulations to all involved in the lab's latest Chemical Science publication. Read the full article here